Factors Influencing Agrobacterium-mediated Transformation of Brassica rapa L.
نویسندگان
چکیده
منابع مشابه
Agrobacterium-mediated transformation of Brassica rapa with a bacterial isochorismate synthase (ICS) gene
The entC gene from Escherichia coli which codes for isochorismate synthase (ICS) was introduced into Brassica rapa varieties i.e. var. rapa cv. Raapstelen and ssp. oleifera. Transgenic plants of ssp. oleifera carrying the ICS gene have been developed via Agrobacterium tumefaciens-mediated transformation. This method involved the use of hypocotyl explants co-cultivated with disarmed Agrobacteriu...
متن کاملAgrobacterium Mediated Transformation of Maize (Zea mays L.)
Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...
متن کاملAgrobacterium–mediated Transformation and Regeneration of Fertile Transgenic Plants of Rapeseed (Brassica napus L.)
A method for transformation of B.napus is presented in this study. The protocol is based on infection of cotyledonary petioles of 5-day-old and hypocotyl segments of 14-day-old seedlings with an Agrobacterium tumefaciens strain LBA 4404 carrying a disarmed binary vector pBI121. The T-DNA region of this binary vector contains the neomycin phosphotransferase II (NPTII) and glucuronidase (GUS) gen...
متن کاملFactors Affecting Agrobacterium-mediated Transformation of Plants
Plant transformation technology has become a versatile platform for cultivar improvement as well as for studying gene function in plants. The development of an efficient method for genetic transformation is a prerequisite for the application of molecular biology to the improvement of a given crop species. Agrobacterium-mediated genetic transformation is the dominant technology used for the prod...
متن کاملHigh-Efficiency Agrobacterium-Mediated Transformation of Tobacco (Nicotiana tabacum)
To improve Agrobacterium-mediated transformation of tobacco, factors influencing gene delivery, including genotype of the plant, bacterial strain, and Agrobacterium transformation procedure, were tested via direct somatic embryogenesis. Leaf tissue of three different tobacco genotypes (Nicotiana tabacum L. cvs. Samsun, and Xanthi, and N. benthamiana) we...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Japanese Journal of Breeding
سال: 1997
ISSN: 0536-3683,2185-291X
DOI: 10.1270/jsbbs1951.47.127